Ingeniería de tejidos dentarios duros in situ y rehabilitación terapéutica  adhesiva mínimamente invasiva

Investigador principal

Doctorandos

Elisabet Palazón Radford

Miembros externos

David H Pashley (Georgia Regents University,Augusta, USA),Franklin Tay (Georgia Regents University, Augusta, USA),Manuel Toledano Pérez (Universidad de Granada),Raquel Osorio (Universidad de Granada),Víctor Feitosa (Universidade Federal do Ceará. Brazil),Lidiany Rodrigues (Universidade Federal do Ceará, Brazil),Lidiany Rodrigues (Universidade federal do Caerá, Brazil), David Gillam (Queen Mary, University of London),Timothy F. Watson (King's College London),Francesco Mannocci (King's College London), Arzu Tezvergil-Mutluay (Institute of Dentistry, University of Turku, Finland),Angelo Putignano (Polytechnic of Marche, School of Dentistry, Ancona, Italy),Delia Brauer (Otto-Schott-Institut Friedrich-Schiller-Universität Jena, Germany), Marcelo Giannini (Universidade UNICAMP de Piracicaba, Brasil), Mário A. Sinhoreti (Universidade UNICAMP de Piracicaba, Brasil),Jeffrey.W.Stansbury (Biomaterials Research Center, University of Colorado, USA)

Líneas de investigación

Área conocimiento ANEP: Tecnología médica y  Patología bucal

Códigos UNESCO: 3314.99 (Biomateriales Dentales); 3207.99 (Tejidos duro dentales)

Nombre: Ingeniería de tejidos dentarios duros in situ por biomateriales adhesivo inteligentes que restablece las propiedades biomecánicas de los tejidos dentarios desmineralizados.

Palabras clave: Adhesión, Bioingeniería, Bioactivo, Biomimético, Colágeno, Degradación, Dentina, Polímeros, In-situ, Tejidos Dentarios, Remineralización, Restauración.             

Resumen: La caries dental es una enfermedad que depende del estilo de vida y de los hábitos adquiridos, con un componente bacteriano, que afecta a los tejidos duros dentales con un índice de casi  un 80% en los niños y el 90% en los adultos. Por ejemplo, en Estados Unidos y también in España, se gastan aproximadamente 100 millones de Euros anualmente en el servicio dental particular. In Inglaterra y Gales los gastos de la seguridad social para el tratamiento de esta enfermedad pueden llegar a costar más de 100 millones de esterlinas por año.  

En la desmineralización de la dentina aparte de la caries un papel muy importante juegan los sistemas adhesivos de resina. La adhesión entre la resina del adhesivo y la dentina se logra a través de la retención micromecánica por la penetración del adhesivo entre las fibras de colágeno expuestas en la dentina desmineralizada parcial o totalmente. Esta desmineralización que expone las fibras de colágeno se consigue mediante el acondicionamiento de la dentina con ácidos o monómeros  ácidos que se encuentran dentro de los sistemas de adhesión de auto-acondicionamiento. 

En odontología restauradora, más del 70% de los tratamientos consiste en  la sustitución y la reparación de las restauraciones existentes en boca porque la adhesión de los materiales restauradores a los tejidos duros se degrada con el paso del tiempo.  Esto se debe a la utilización de materiales sintéticos biocompatibles. Esto es debido a la utilización de materiales sintéticos, incompatibles que se aplican en un entorno biológico que no es el idóneo para su colocación siempre después de una eliminación ultraconservadora de la caries a la cual se le junta la no utilización de métodos biomiméticos que ayudan la adhesión, la  conservación o incluso a veces mejorar, el tejido reparado. Los materiales restauradores que liberan iones, cuando se aplican, solo pueden recuperar una parte de la funcionalidad biomecánica de la dentina cariada  debido a la pobre asociación mineral a nivel intrafibrilar. La recuperación total de la funcionalidad biomecánica de la dentina solo se puede conseguir después de la remineralización biomimética intrafibrilar con técnica de ingeniería tisular in situ. Si esta condición no se cumple el proceso de bio-mineralización debe considerarse incompleto. Además, las fibras de colágeno que se han remineralizado de forma inadecuada sufren una degradación por las bacterias o la activación  por parte del ácido de metaloproteinasas endogenas (MMPs) que se encuentran en la dentina.

Este será el primer proyecto en utilizar conceptos de ingeniería tisular in situ de bio-mineralización de grandes cavidades en los dientes y  superar los problemas que existen durante la restauración de grandes destrucciones cariosas mediante procedimientos de rehabilitación adhesiva mínimamente invasivos. Los clínicos serán capaces de aplicar sistemas adhesivos durante los tratamientos que serán capaces de biomineralizar la dentina, que alargaran la vida de las restauraciones de composite. Esto mejorará la calidad de las restauraciones dentales en clínica aumentando la rentabilidad del tratamiento.

Los objetivos de nuestra investigación serán de generar metodología innovadoras de ingeniería de tejidos in situ por biomateriales adhesivo inteligentes que restablece las propiedades biomecánicas de los tejidos dentarios desmineralizados in conjunción con procederás de rehabilitación dentales mínimamente invasiva. 

Esta técnica será capaz de:

  • Liberar iones y componentes biomiméticos 
  • Formar precursores de nano-nucleación de apatita en vez de micro- aglomerados.
  • Bio-remineralización jerárquica  de colágeno extra e intrafibrilar.
  • Inducir diferenciación de células estaminales células a los odontoblastos/fibroblastos.
  • Presentar una actividad antibacteriana en biofilm órale cariogenico
  • Recuperación de la funcionalidad biomecánica de las interfases adhesivas desmineralizadas alargando la vida útil de las restauraciones dentales.

Name: In Situ dental tissues engineering and therapeutic minimally invasive adhesive rehabilitation  

Key Words: Adhesion, Bio-engineering, Bioactive, Biomimetic, Collagen, Degradation, Dentine, Polymers In-situ; Remineralization, Rehabilitation

Summary: Dental caries is a behavioural, lifestyle disease with a bacterial component, affecting the dental hard tissues of almost 80% of children and 90% of adults. For instance, In the United States alone, more than 100 million dollars is spent annually on dental service, while the treatment of dental caries in England and Wales costs the NHS more than 100 million per annum. Apart from caries, resin-dentine bonding is another major reason for dentine demineralisation. The formation of resin-dentin bonds is accomplished predominantly by micromechanical retention via resin penetration and entanglement of exposed collagen fibrils in the partially or completely demineralized dentin. This is achieved by etching dentin with acids or acidic resin monomers derived from self-etching primers/adhesives to expose the collagen fibrils. Unfortunately, more than 70% of all restorative dentistry comprises of replacement/repair of existing restorations, as bonding to dental hard tissues still suffers from long-term interfacial failure. This is due to the use of synthetic, bio-incompatible materials placed in a hostile biological environment subsequent to ultra-conservative caries excavation, without using therapeutic and biomimetic methods to bond and maintain, or even improve, the repaired tissue.

Commonly-used ion-releasing restorative materials may only recover a small part of the biomechanical functionality of the carious dentine due to poor mineral association at the intrafibrillar level. The total re-establishment of biomechanical dentine functionality is only possible subsequent to intrafibrillar biomimetic remineralisation: if this condition is not satisfied then the biomineralisation process must be considered incomplete. Furthermore, inadequately remineralised collagen fibrils undergo degradation by bacterial or acid-activated dentine endogenous matrix metalloproteinases (MMPs).

This will be the first project to use high-impact In Situ bio-engineered concepts for bio-mineralise and restoring large cavities in teeth, overcoming existing problems that cannot be adequately addressed during the restoration of large carious dentine cavities with minimally invasive procedures. Clinicians will be able to apply therapeutic bonding materials capable of biomineralising carious dentine providing a durable foundation for resin composite restorations. This will improve the quality of dental restorations in routine practice, promoting cost-effective care.

The main aims of this research group focus on the generation of revolutionary techniques based on In Situ dental tissue engineering concepts and using smart resin-based biomaterials able to reestablish the biomechanical properties of mineral-depleted dental hard tissues in conjunction with minimally invasive dentistry.

These innovative approaches will be able to induce:

- Apatite nano-nucleation precursors (ANP) rather than micro-cluster agglomeration

- Hierarchical collagen bioremineralisation at intra- and extra-fibrillar level

- Differentiation of pulpal cells into reparative odontoblasts/fibroblast-like cells

- Bacteriostatic and/or antibacterial activity on caries biofilms

- Recovery of the biomechanical functionality of mineral-depleted bonded interfaces providing a long-term foundation for minimally invasive dental restorations.  

Título del proyecto: Effect of mechanical load cycling on the interface morphology and microtensile bond strength of an experimental light-curing glass ionomer cement restorative material in applied after burs or bioglass air-abrasion treatment 

Investigador responsable: Salvatore Sauro

Entidad financiadora: VOCO

Duración - Enero 2017 - Julio 2017

 

Título del proyecto: Premio Gaceta Dental - Remineralización biomimética y biocompatibilidad de un sistema de curado por luz con microrrelleno bioactivo embebido con ácido 4-oxoheptanodioico

Investigador responsable: Salvatore Sauro, Arlinda Luzi.

Entidad financiadora: Gaceta dental

Duración – Septiembre 2014

 

Título del proyecto: Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016, de la Secretaría de Estado de Investigación, Desarrollo e Innovación

Investigador responsable: Salvatore Sauro, Arlinda Luzi.

Entidad financiadora: CEU.UCH

Duración – desde 31 2015 agosto hasta 15 Septiembre 2015

 

Título del proyecto: Consolidación de Indicadores CEU-UCH 2015-2016; Ref. INDI1527.

Investigador responsable: Salvatore Sauro, Arlinda Luzi.

Entidad financiadora: CEU.UCH

Duración – desde 31 Agosto 2016 hasta 15 Septiembre 2016

 

  1. Tezvergil-Mutluay A; Seseogullari-Dirihan R; Feitosa VP; Cama G; Brauer DS; Sauro S. Effects of Composites Containing Bioactive Glasses on Demineralized  Dentin.05/2017. Journal of Dental Research 2017.
  1. Rodrigues N, Costa de Souza L, Feitosa V, Loguercio A, D'Arcangelo C, Sauro S, Saboia V. Effect of different conditioning/deproteinization protocols on the bond strength and degree of conversion of self-adhesive resin cements applied to dentin. [Epub ahead of print]. 2017 - http://doi.org/10.1016/j.ijadhadh.2017.03.013.
  1. Bagheri M, Pilecki P, Sauro S, Sherriff M, Watson TF, Hosey MT. An in vitro investigation of pre-treatment effects before fissure sealing. Int J Paediatr Dent. 2017 Feb 10. doi: 10.1111/ipd.12290. [Epub ahead of print].
  1. Alhashimi RA, Mannocci F, Sauro S. Bioactivity, cytocompatibility and thermal properties of experimental Bioglass-reinforced composites as potential root-canal filling materials. J Mech Behav Biomed Mater. 2017 Jan 15;69:355-361. doi: 10.1016/j.jmbbm.2017.01.022.
  1. Enrico Conserva; Ugo Consolo; Alberto Gimenez Sancho; Federico Foschi; Gaetano Paolone; Massimo Giovarrusscio; Salvatore Sauro. Stress distribution in carbon-post applied with different composite core materials: a three-dimensional finite element analysis. Journal of Adhesion Science and Technology. 03/2017.
  1. Abedi-Amin A, Luzi A, Giovarruscio M, Paolone G, Darvizeh A, Agulló VV, Sauro S. Innovative root-end filling materials based on calcium-silicates and calcium-phosphates. J Mater Sci Mater Med. 2017;28(2):31. doi: 10.1007/s10856-017-5847-1.
  1. Sfalcin RA, Correr AB, Morbidelli LR, Araújo TG, Feitosa VP, Correr-Sobrinho L, Watson TF, Sauro S. Influence of bioactive particles on the chemical-mechanical properties of experimental enamel resin infiltrants. Clin Oral Investig. 2016 Nov 12. [Epub ahead of print]
  1. Akram, Z., Abduljabbar, T., Sauro, S., Daood, U. Effect of photodynamic therapy and laser alone as adjunct to scaling and root planing on gingival crevicular fluid inflammatory proteins in periodontal disease: A systematic review. Photodiagnosis Photodyn Ther. 2016 Dec;16:142-153.  doi: 10.1016/j.pdpdt.2016.09.004.
  1. Abuna, G., Feitosa, V.P., Correr A.B., Cama, G., Giannini, M., Sinhoreti, M.A., Pashley, D.H., Sauro, S. Bonding performance of experimental bioactive/biomimetic self-etch adhesives doped with calcium-phosphate fillers and biomimetic analogs of phosphoproteins. J Dent. 2016 Sep;52:79-86. doi: 10.1016/j.jdent.2016.07.016. Epub 2016 Jul 27.
  1. Salvatore Sauro; David H. Pashley. Strategies to stabilise dentine-bonded interfaces through remineralising operative approaches – State of The Art. International Journal of Adhesion and Adhesives. 09/2016.
  1. Najeeb, S., Khurshid, Z., Zafar, M.S., Khan, A.S., Zohaib, S., Martí, J.M., Sauro, S., Matinlinna, J.P., Rehman, I.U. Modifications in Glass Ionomer Cements: Nano-Sized Fillers and Bioactive Nanoceramics. Int J Mol Sci. 2016 Jul 14;17(7). pii: E1134. Doi: 10.3390/ijms17071134. Review.
  1. Degrazia, F.W., Leitune, V.C., Takimi, A.S., Collares, F.M., Sauro, S. Physicochemical and bioactive properties of innovative resin-based materials containing functional halloysite-nanotubes fillers.  Dent Mater. 2016 Sep;32(9):1133-43.  Doi: 10.1016/j.dental.2016.06.012. Epub 2016 Jul 6.
  1. Sauro, S., Lin, C.Y., Bikker, F.J., Cama, G, Dubruel, P., Soria, J.M., D''Onofrio, A., Gillam, D. Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules. Caries Res. 2016;50(3):303-9. Doi: 10.1159/000445444..
  1. Andrade Neto, D.M., Carvalho, E.V., Rodrigues, E.A., Feitosa, V.P., Sauro, S., Mele, G., Carbone, L., Mazzetto, S.E., Rodrigues, L.K., Fechine, P.B.A. Novel hydroxyapatite nanorods improve anti-caries efficacy of enamel infiltrants. Dental Materials, 2016; 32 (6), pp. 784-793.  DOI: 10.1016/j.dental.2016.03.026
  1. Alhashimi, R.A., Mannocci, F., Sauro, S. Experimental polyethylene-hydroxyapatite carrier-based endodontic system: An in vitro study on dynamic thermomechanical properties, sealing ability, and measurements of micro-computed tomography voids (2016) European Journal of Oral Sciences, 124 (3), pp. 279-286. DOI: 10.1111/eos.12265
  1. Sauro, S., Lin, C.-Y., Bikker, F.J., Cama, G., Dubruel, P., Soria, J.M., D'Onofrio, A., Gillam, D. Di-Calcium Phosphate and Phytosphingosine as an Innovative Acid-Resistant Treatment to Occlude Dentine Tubules (2016) Caries Research, pp. 303-309. DOI: 10.1159/000445444
  1. Rocamonde, B., Paradells, S., Garcia Esparza, M.A., Vives, M.S., Sauro, S., Ramos, C.M., Pradas, M.M., Soria, J.M. Combined application of polyacrylate scaffold and lipoic acid treatment promotes neural tissue reparation after brain injury (2016) Brain Injury, 30 (2), pp. 208-216. DOI: 10.3109/02699052.2015.1091505
  1. Sauro, S., Pashley, D.H. Strategies to stabilise dentine-bonded interfaces through remineralising operative approaches - State of The Art (2016) International Journal of Adhesion and Adhesives, . Article in Press.  DOI: 10.1016/j.ijadhadh.2016.03.014
  1. D’Alpino, P.H.P., Svizero, N.D.R., Bim Júnior, O., Valduga, C.J., Graeff, C.F.D.O., Sauro, S. Effects of age condition on the distribution and integrity of inorganic fillers in dental resin composites (2015) Clinical Oral Investigations, DOI: 10.1007/s00784-015-1599-9
  1. Darvizeh, A., Luzi, A., Amin, A.A., Oliveira-Ogliari, A., Ogliari, F.A., Feitosa, V.P., García-Esparza, M.A., Pascual, A., Sauro, S. In-situ nano-silica deposition and air-abrasion with Bioglass 45S5 or aluminium oxide: Effects on methacrylate bonding to yttria-tetragonal zirconia polycrystal (2015) International Journal of Adhesion and Adhesives, 62, art. no. 1669, pp. 32-39.  DOI: 10.1016/j.ijadhadh.2015.06.008
  1. Sauro, S., Osorio, R., Watson, T.F., Toledano, M. Influence of phosphoproteins' biomimetic analogs on remineralization of mineral-depleted resin-dentin interfaces created with ion-releasing resin-based systems (2015) Dental Materials, 31 (7), pp. 759-777. DOI: 10.1016/j.dental.2015.03.013
  1. Bacchi, A., Abuna, G., Consani, R.L., Sinhoreti, M.A., Sauro, S., Feitosa, V.P. Effects of simulated pulpal pressure, mechanical and thermocycling challenge on the microtensile bond strength of resin luting cements (2015) International Journal of Adhesion and Adhesives, 60, pp. 69-74. DOI: 10.1016/j.ijadhadh.2015.03.009
  1. Stramazzotti, D., Coiana, C., Zizzi, A., Spazzafumo, L., Sauro, S., D'Angelo, A.B., Rubini, C., Aspriello, S.D. Impact of smoking on guided tissue regeneration using a biocomposite poly (lactic-co-glycolic) acid/sub-micron size hydroxyapatite with a rubber dam as an alternative barrier (2015) International Journal of Immunopathology and Pharmacology, 28 (1), pp. 21-28. DOI: 10.1177/0394632015573159
  1. Oliveira-Ogliari, A., Collares, F.M., Feitosa, V.P., Sauro, S., Ogliari, F.A., Moraes, R.R. Methacrylate bonding to zirconia by in situ silica nanoparticle surface deposition (2015) Dental Materials, 31 (1), pp. 68-76. DOI: 10.1016/j.dental.2014.11.011
  1. Oliveira-Ogliari, A., Collares, F.M., Feitosa, V.P., Sauro, S., Ogliari, F.A., Moraes, R.R. Methacrylate bonding to zirconia by in situ silica nanoparticle surface deposition (2015) Dental Materials, 31 (1), pp. 68-76. DOI: 10.1016/j.dental.2014.11.011
  1. Osorio, R., Sauro, S., Watson, T.F., Toledano, M. Polyaspartic acid enhances dentine remineralization bonded with a zinc-doped Portland-based resin cements. 2015) International Endodontic Journal. DOI: 10.1111/iej.12518
  1. Padovani, G.C., Feitosa, V.P., Sauro, S., Tay, F.R., Durán, G., Paula, A.J., Durán, N. Advances in Dental Materials through Nanotechnology: Facts, Perspectives and Toxicological Aspects (2015) Trends in Biotechnology, 33 (11), pp. 621-636. Cited 1 time. DOI: 10.1016/j.tibtech.2015.09.005
  1. Sauro, S., Osorio, R., Watson, T.F., Toledano, M. Influence of phosphoproteins' biomimetic analogs on remineralization of mineral-depleted resin-dentin interfaces created with ion-releasing resin-based systems (2015) Dental Materials, DOI: 10.1016/j.dental.2015.03.013
  1. Wang, Z., Shen, Y., Haapasalo, M., Wang, J., Jiang, T., Wang, Y., Watson, T.F., Sauro, S. Polycarboxylated microfillers incorporated into light-curable resin-based dental adhesives evoke remineralization at the mineral-depleted dentin (2014) Journal of Biomaterials Science, Polymer Edition, 25 (7), pp. 679-697. DOI: 10.1080/09205063.2014.891926
  1. Feitosa, V.P., Pomacóndor-Hernández, C., Ogliari, F.A., Leal, F., Correr, A.B., Sauro, S. Chemical interaction of 10-MDP (methacryloyloxi-decyl-dihydrogen-phosphate) in zinc-doped self-etch adhesives (2014) Journal of Dentistry, 42 (3), pp. 359-365. DOI: 10.1016/j.jdent.2014.01.003
  1. Mestres, G., Aguilera, F.S., Manzanares, N., Sauro, S., Osorio, R., Toledano, M., Ginebra, M.P. Magnesium phosphate cements for endodontic applications with improved long-term sealing ability (2014) International Endodontic Journal, 47 (2), pp. 127-139. DOI: 10.1111/iej.12123
  1. Feitosa, V.P., Ogliari, F.A., Van Meerbeek, B., Watson, T.F., Yoshihara, K., Ogliari, A.O., Sinhoreti, M.A., Correr, A.B., Cama, G., Sauro, S. Can the hydrophilicity of functional monomers affect chemical interaction? (2014) Journal of Dental Research, 93 (2), pp. 201-206. DOI: 10.1177/0022034513514587
  1. Tezvergil-Mutluay, A., Seseogullari-Dirihan, R., Feitosa, V.P., Tay, F.R., Watson, T.F., Pashley, D.H., Sauro, S. Zoledronate and ion-releasing resins impair dentin collagen degradation (2014) Journal of Dental Research, 93 (10), pp. 999-1004. DOI: 10.1177/0022034514546043
  1. Toledano, M., Osorio, E., Aguilera, F.S., Sauro, S., Cabello, I., Osorio, R. In vitro mechanical stimulation promoted remineralization at the resin/dentin interface (2014) Journal of the Mechanical Behaviour of Biomedical Materials, 30, pp. 61-74. DOI: 10.1016/j.jmbbm.2013.10.018
  1. Zavattini, A., Feitosa, V.P., Mannocci, F., Foschi, F., Babbar, A., Luzi, A., Ottria, L., Mangani, F., Casula, I., Sauro, S. Bonding ability of experimental resin-based materials containing (ion-releasing)-microfillers applied on water-wet or ethanol-wet root canal dentine (2014) International Journal of Adhesion and Adhesives, 54, pp. 214-223. DOI: 10.1016/j.ijadhadh.2014.06.007
  1. Feitosa, V.P., Sauro, S., Ogliari, F.A., Ogliari, A.O., Yoshihara, K., Zanchi, C.H., Correr-Sobrinho, L., Sinhoreti, M.A., Correr, A.B., Watson, T.F., Van Meerbeek, B. Impact of hydrophilicity and length of spacer chains on the bonding of functional monomers (2014) Dental Materials, 30 (12), pp. e317-e323. Cited 6 times. DOI: 10.1016/j.dental.2014.06.006
  1. Osorio, R., Yamauti, M., Sauro, S., Watson, T.F., Toledano, M. Zinc incorporation improves biological activity of beta-tricalcium silicate resin-based cement (2014) Journal of Endodontics, 40 (11), pp. 1840-1845. DOI: 10.1016/j.joen.2014.06.016
  1. Toledano, M., Aguilera, F.S., Sauro, S., Cabello, I., Osorio, E., Osorio, R. Load cycling enhances bioactivity at the resin-dentin interface (2014) Dental Materials, 30 (7), pp. e169-e188. DOI: 10.1016/j.dental.2014.02.009
  1. Feitosa, V.P., Sauro, S., Ogliari, F.A., Stansbury, J.W., Carpenter, G.H., Watson, T.F., Sinhoreti, M.A., Correr, A.B. The role of spacer carbon chain in acidic functional monomers on the physicochemical properties of self-etch dental adhesives (2014) Journal of Dentistry, 42 (5), pp. 565-574. DOI: 10.1016/j.jdent.2014.02.009
  1. Gotti, V.B., Feitosa, V.P., Sauro, S., Correr-Sobrinho, L., Correr, A.B. Indirect resin composite restorations bonded to dentin using self-adhesive resin cements applied with an electric current-assisted method (2014) American Journal of Dentistry, 27 (5), pp. 233-236.
  1. Sauro, S., Osorio, R., Fulgêncio, R., Watson, T.F., Cama, G., Thompson, I., Toledano, M. Erratum: Remineralisation properties of innovative light-curable resin-based dental materials containing bioactive micro-fillers (Journal of Materials Chemistry B (2013) 1 (2624-2638). Journal of Materials Chemistry B, 1 (48), p. 6670. DOI: 10.1039/c3tb90147e
  1. Feitosa, V.P., Bazzocchi, M.G., Putignano, A., Orsini, G., Luzi, A.L., Sinhoreti, M.A.C., Watson, T.F., Sauro, S. Dicalcium phosphate (CaHPO4·2H2O) precipitation through ortho- or meta-phosphoric acid-etching: Effects on the durability and nanoleakage/ultra-morphology of resin-dentine interfaces (2013) Journal of Dentistry, 41 (11), pp. 1068-1080. DOI: 10.1016/j.jdent.2013.08.014
  1. Levrini, L., Abbate, G.M., Migliori, F., Orrù, G., Sauro, S., Caprioglio, A. Assessment of the periodontal health status in patients undergoing orthodontic treatment with fixed or removable appliances. A microbiological and preliminary clinical study (2013) Cumhuriyet Dental Journal, 16 (4), pp. 296-307. DOI: 10.7126/cdj.2013.1974
  1. Feitosa, V.P., Watson, T.F., Vitti, R.P., Bacchi, A., Correr-Sobrinho, L., Correr, A.B., Sinhoreti, M.A.C., Sauro, S. Prolonged curing time reduces the effects of simulated pulpal pressure on the bond strength of one-step self-etch adhesives (2013) Operative Dentistry, 38 (5), pp. 545-554. DOI: 10.2341/12-180-L
  1. Toledano, M., Sauro, S., Cabello, I., Watson, T., Osorio, R. A Zn-doped etch-and-rinse adhesive may improve the mechanical properties and the integrity at the bonded-dentin interface (2013) Dental Materials, 29 (8), pp. e142-e152. Cited 22 times. DOI: 10.1016/j.dental.2013.04.024
  1. Profeta, A.C., Mannocci, F., Foxton, R., Watson, T.F., Feitosa, V.P., De Carlo, B., Mongiorgi, R., Valdré, G., Sauro, S. Experimental etch-and-rinse adhesives doped with bioactive calcium silicate-based micro-fillers to generate therapeutic resin-dentin interfaces (2013) Dental Materials, 29 (7), pp. 729-741. DOI: 10.1016/j.dental.2013.04.001
  1. Sauro, S., Osorio, R., Osorio, E., Watson, T.F., Toledano, M. Novel light-curable materials containing experimental bioactive micro-fillers remineralise mineral-depleted bonded-dentine interfaces (2013) Journal of Biomaterials Science, Polymer Edition, 24 (8), pp. 940-956. DOI: 10.1080/09205063.2012.727377
  1. Sauro, S., Osorio, R., Fulgêncio, R., Watson, T.F., Cama, G., Thompson, I., Toledano, M. Remineralisation properties of innovative light-curable resin-based dental materials containing bioactive micro-fillers (2013) Journal of Materials Chemistry B, 1 (20), pp. 2624-2638. DOI: 10.1039/c3tb00205e